16180 4932418E24Rik Status: Cancelled
| Gene | 4932418E24Rik |
| Chromosome | 2 |
| Deletion Start | 26,294,380 |
| Deletion End | 26,271,739 |
| Deletion Size | 22,642 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 26,293,737 | 26,293,759 | TCCACCTCAAACAGTTCCCTATG |
| TUP | 26,293,712 | 26,293,736 | TGGCCTGGTCTGGCAATATCAAGGA |
| TUR | 26,293,636 | 26,293,655 | CGAGCTGTCCACGTCCTTAG |
| TDF | 26,272,687 | 26,272,708 | GCCTCTGGGAGAAAGTAGAGTG |
| TDP | 26,272,661 | 26,272,684 | CAGAGGCTGTGTTCACCGCCTACA |
| TDR | 26,272,608 | 26,272,631 | TGCAGGGATTAGAAGGCTTTGAAG |
PCR Primers
| Primer | Sequence |
| SU | TCTCTGTTGGGTGTGTGCTC |
| SD | ATGCCTTATACCCATGCCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 124 bp | + | + | - |
| TDF | TDR | 101 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
