16218 1110038F14Rik Status: ES cell colonies screened / QC positives
| Gene | 1110038F14Rik |
| Chromosome | 15 |
| Deletion Start | 76,780,889 |
| Deletion End | 76,779,242 |
| Deletion Size | 1,648 |
| Genome Build | 37 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 76,779,425 | 76,779,444 | GCGCTGAAGGTATGGGAGTG |
| TUP | 76,779,448 | 76,779,468 | TCTGCGGGACCCTGTTACCCA |
| TUR | 76,779,485 | 76,779,502 | TCCGCCTGGGACAGAAAG |
| TDF | 76,780,709 | 76,780,730 | GTGAGGCCACCAGAAAGAAGAG |
| TDP | 76,780,734 | 76,780,755 | AAGGGTCTGCAGGCCTCGTCCT |
| TDR | 76,780,825 | 76,780,844 | ATGAGCGGTCCAGCATGTAG |
PCR Primers
| Primer | Sequence |
| SU | ATCCACCCAAGTGCTTTCTG |
| SD | GCCTCCTCTAACACATTCGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 136 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16218A-A6 | VGB6 | C57BL/6NTac | |
| 16218A-C4 | VGB6 | C57BL/6NTac | |
| 16218A-G6 | VGB6 | C57BL/6NTac | |
| 16218A-F1 | VGB6 | C57BL/6NTac | |
