16253 Ccdc14 Status: ES cell colonies screened / QC no positives
| Gene | Ccdc14 |
| Chromosome | 16 |
| Deletion Start | 34,723,935 |
| Deletion End | 34,690,661 |
| Deletion Size | 33,275 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 34,690,989 | 34,691,007 | GAGCGCAGTTTGGTGTCAG |
| TUP | 34,691,011 | 34,691,031 | CCGGGTTCGAAGCCCAGCCTT |
| TUR | 34,691,050 | 34,691,070 | AAAGCACAGAGCGGGAAGGAG |
| TDF | 34,723,521 | 34,723,543 | GCACAGACCTCTAAAGGAGAAAG |
| TDP | 34,723,545 | 34,723,567 | AAGCCACGTGCACCTGCAGTTTG |
| TDR | 34,723,587 | 34,723,605 | GATCTCCGGGTGCATCTTC |
PCR Primers
| Primer | Sequence |
| SU | TGCCATCCTTTATCAACAGC |
| SD | AATGTTAATGCAGGCCATCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
