16274 Habp2 Status: ES cell colonies screened / QC no positives
| Gene | Habp2 |
| Chromosome | 19 |
| Deletion Start | 56,319,717 |
| Deletion End | 56,287,999 |
| Deletion Size | 31,719 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 56,288,841 | 56,288,861 | CCCAAGCCTCCAAGAAATCAC |
| TUP | 56,288,865 | 56,288,892 | CAACCAACTGCTCTTGTGGTAGAGGTCA |
| TUR | 56,288,909 | 56,288,926 | TGGAAGCCGCCATGTCTG |
| TDF | 56,319,077 | 56,319,094 | TGCTCAGCAGGCAGTAGG |
| TDP | 56,319,103 | 56,319,130 | ATAGACTACCTCTGGGTCTCTGCCGTAC |
| TDR | 56,319,149 | 56,319,168 | GGTGGGTGATGGCTTGTTTG |
PCR Primers
| Primer | Sequence |
| SU | CTCAGAGGTGGCAGAGGTTC |
| SD | TGAGAATGGCTGGTTCTGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
