16277 Tha1 Status: ES cell colonies screened / QC positives
| Gene | Tha1 |
| Chromosome | 11 |
| Deletion Start | 117,734,710 |
| Deletion End | 117,729,733 |
| Deletion Size | 4,978 |
| Genome Build | 37 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 117,734,135 | 117,734,157 | CCACATCAGGAACAGGGAAGAAC |
| TUP | 117,734,101 | 117,734,129 | TTTGAGACAGGTCTTACTACAAGGAGGGC |
| TUR | 117,734,074 | 117,734,097 | CAGCCAATGAATGAAGGAGAGTAG |
| TDF | 117,730,351 | 117,730,370 | GGTGCAACCAGCAGAGTCAG |
| TDP | 117,730,326 | 117,730,349 | AACCAGGCCAGAGTAGAGCCTCCA |
| TDR | 117,730,259 | 117,730,277 | GGGCCTCAGATGTGGGAAC |
PCR Primers
| Primer | Sequence |
| SU | TTTCCCAGAGTGGCTGAGAC |
| SD | CAGTCCACCTCTGACAACTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 112 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16277A-A9 | VGB6 | C57BL/6NTac | |
| 16277A-A11 | VGB6 | C57BL/6NTac | |
| 16277A-C10 | VGB6 | C57BL/6NTac | |
| 16277A-H12 | VGB6 | C57BL/6NTac | |
| 16277A-B10 | VGB6 | C57BL/6NTac | |
| 16277A-D2 | VGB6 | C57BL/6NTac | |
