16294 Cacna1e Status: Cancelled
| Gene | Cacna1e |
| Chromosome | 1 |
| Deletion Start | 154,725,916 |
| Deletion End | 154,701,280 |
| Deletion Size | 24,637 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 154,725,210 | 154,725,232 | AGTCCCTCTGCTCCCATTCCTAC |
| TUP | 154,725,185 | 154,725,209 | CAGCTGGGAAGACACTTGCACTGTC |
| TUR | 154,725,149 | 154,725,171 | CAAATCCCTCCCAGAACCTCAAC |
| TDF | 154,701,387 | 154,701,409 | GAACTTGGTCCTTAACACCTCTG |
| TDP | 154,701,357 | 154,701,384 | TCACGCCATTTGAGTACATGATCCTGGC |
| TDR | 154,701,332 | 154,701,353 | GACGATGCAGTTGGCAATGATG |
PCR Primers
| Primer | Sequence |
| SU | TGGTACGTGTGCCAGAAGAG |
| SD | CACTCGGCTTTCACCATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
