Gene | Fam54a |
Chromosome | 10 |
Deletion Start | 20,360,638 |
Deletion End | 20,347,914 |
Deletion Size | 12,725 |
Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
Primer | Start | End | Sequence |
TUF | 20,348,629 | 20,348,651 | CCAATGGGAGGAAGTTAGGACAG |
TUP | 20,348,659 | 20,348,685 | AAACAAAGCAGGAACCTGGAGGCAGGA |
TUR | 20,348,703 | 20,348,725 | GCTGGTGGAACAAGTCAGTTATC |
TDF | 20,359,903 | 20,359,920 | CCTGGCAATCCAGCTCAC |
TDP | 20,359,921 | 20,359,945 | TCTCATGCCTGGAGTCTGGTGTCTG |
TDR | 20,359,952 | 20,359,971 | CAAGGCAGACAGTGGCTCAG |
PCR Primers
Primer | Sequence |
SU | GCTTGACACCTTACTGGCA |
SD | AGGAGCCTCAGTAGGAAGCC |
LacInR | TTGACTGTAGCGGCTGATGTTG |
LacInf | GGTAAACTGGCTCGGATTAGGG |
NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
NeoInF | TTCGGCTATGACTGGGCACAACAG |
LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
Forward Primer | Reverse Primer | Length | WT | HET | KO |
SU | LacZRev | bp | - | + | + |
TUF | TUR | 97 bp | + | + | - |
TDF | TDR | 69 bp | + | + | - |
NeoFwd | SD | bp | - | + | + |
LacInf | LacInR | 210 bp | - | + | + |
NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
16305A-D5 | VGB6 | C57BL/6NTac | |
16305E-A6 | VGB6 | C57BL/6NTac | |