16370 Etv1 Status: ES cell colonies screened / QC no positives
| Gene | Etv1 |
| Chromosome | 12 |
| Deletion Start | 38,783,281 |
| Deletion End | 38,780,938 |
| Deletion Size | 2,344 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 38,781,578 | 38,781,597 | CATGCTCTGGGAAGCAACTC |
| TUP | 38,781,599 | 38,781,619 | CCCTCCTCACTGCCTGCCGAA |
| TUR | 38,781,651 | 38,781,675 | CAGGAGAACTCCAACACATATTGTC |
| TDF | 38,783,177 | 38,783,198 | TCATGTGATGAAGCCTACAGAC |
| TDP | 38,783,199 | 38,783,226 | TGTTGAGTCTCATGTTGGTGTGTCTGAC |
| TDR | 38,783,258 | 38,783,281 | AGCAAGCCATGTTTCTTGTAACTG |
PCR Primers
| Primer | Sequence |
| SU | AAGCAAGTCTCGTTGATCGC |
| SD | TGAGGTCTCTTGAGGTAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 98 bp | + | + | - |
| TDF | TDR | 105 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
