16452 Tle4 Status: ES cell colonies screened / QC no positives
| Gene | Tle4 |
| Chromosome | 19 |
| Deletion Start | 14,597,409 |
| Deletion End | 14,564,392 |
| Deletion Size | 33,018 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 14,596,548 | 14,596,570 | CCGGTAAACAGAAAAGGAAGCAG |
| TUP | 14,596,505 | 14,596,530 | AGACTTGGAAAACGCTGAGGCCAAAC |
| TUR | 14,596,472 | 14,596,494 | CCAGCCTCCTGACCCAATAAATC |
| TDF | 14,564,616 | 14,564,640 | AAATCCTGCTGTTAATGTGACTCTG |
| TDP | 14,564,592 | 14,564,615 | TTGTCACTGCCTCCCTTAATGGGC |
| TDR | 14,564,544 | 14,564,565 | ACTCTGGCTGGCAACATCTAAG |
PCR Primers
| Primer | Sequence |
| SU | TCATTCCGAGGAATAGCAGC |
| SD | TATCCACTAGGTGAAGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 99 bp | + | + | - |
| TDF | TDR | 97 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
