16491 Nlrp4c Status: ES cell colonies screened / QC no positives
| Gene | Nlrp4c |
| Chromosome | 7 |
| Deletion Start | 6,092,632 |
| Deletion End | 6,060,598 |
| Deletion Size | 32,035 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 6,061,045 | 6,061,068 | AGGGTAGCATGACCTAGCCTATTC |
| TUP | 6,061,073 | 6,061,097 | AAACTTCTACCCAGGACATTAATAA |
| TUR | 6,061,135 | 6,061,158 | AAATCCTCAGTGTTTCCATTTCAC |
| TDF | 6,092,466 | 6,092,487 | GGTTAGTCAACTGCTCCCTCAG |
| TDP | 6,092,488 | 6,092,514 | TGAGCAATGTTGGGACTACCTTTCGGA |
| TDR | 6,092,567 | 6,092,588 | CCTTCAGCCCTTCATCCTTCAG |
PCR Primers
| Primer | Sequence |
| SU | CAGCCCATTGACCCACTC |
| SD | TACACCCTTCTGTCTAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 114 bp | + | + | - |
| TDF | TDR | 123 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
