| Gene | Efemp1 |
| Chromosome | 11 |
| Deletion Start | 28,868,035 |
| Deletion End | 28,854,477 |
| Deletion Size | 13,559 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 28,854,504 | 28,854,521 | ACTCTGGCGCTGGTCAAG |
| TUP | 28,854,522 | 28,854,548 | TCACAGTACACCGAGGAAACCATCACA |
| TUR | 28,854,554 | 28,854,578 | ATCTCAAATGTCATCATGCCTTACC |
| TDF | 28,867,545 | 28,867,565 | GAGCAGACCTCTGTGTTCATC |
| TDP | 28,867,571 | 28,867,596 | TGGTCCTCTTCCTATCCCATGTGTCC |
| TDR | 28,867,669 | 28,867,688 | TGCAAGCATCTGGGACAATG |
PCR Primers
| Primer | Sequence |
| SU | GTATCTTTCGCTGAGCTGGG |
| SD | GTTGGGAACCACTGATGAAT |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 144 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16500B-A10 | VGB6 | C57BL/6NTac | |
| 16500B-H2 | VGB6 | C57BL/6NTac | |
| 16500B-F7 | VGB6 | C57BL/6NTac | |
| 16500B-E6 | VGB6 | C57BL/6NTac | |
| 16500C-E4 | VGB6 | C57BL/6NTac | |
| 16500C-B10 | VGB6 | C57BL/6NTac | |
