16587 Zfp790 Status: ES cell colonies screened / QC no positives
| Gene | Zfp790 |
| Chromosome | 7 |
| Deletion Start | 29,830,259 |
| Deletion End | 29,823,139 |
| Deletion Size | 7,121 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 29,823,916 | 29,823,935 | TTCTGGCGCACTTAGAGGTC |
| TUP | 29,823,937 | 29,823,962 | TGACGGAAACCCTGAGGGTACCAAGA |
| TUR | 29,824,007 | 29,824,025 | GAGCGGAACACGTTGCTTC |
| TDF | 29,829,353 | 29,829,374 | CCCTCAGACCTCTCTCGACATC |
| TDP | 29,829,377 | 29,829,400 | AAGATTCACACGGGCGAGAAGCCC |
| TDR | 29,829,406 | 29,829,428 | AGCCCTTCCCACATTCCTTACAC |
PCR Primers
| Primer | Sequence |
| SU | CAGATCCTGGTCTCTGGAATG |
| SD | GCATGATTTTCCAGGTGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 110 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
