| Gene | Sdc3 |
| Chromosome | 4 |
| Deletion Start | 130,822,810 |
| Deletion End | 130,816,854 |
| Deletion Size | 5,957 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 130,816,976 | 130,816,994 | AGGTCTCCCTGCCCATCTC |
| TUP | 130,816,995 | 130,817,020 | TGATATCTAGCACCAACCCAGGAGCC |
| TUR | 130,817,034 | 130,817,057 | TCCATACAGTGGCAAGACTCATTC |
| TDF | 130,821,877 | 130,821,895 | CCAGCCTTTACCCAAGACC |
| TDP | 130,821,896 | 130,821,919 | TTGAAGGCTTTACCCTGGTGGGAC |
| TDR | 130,821,940 | 130,821,961 | GAGGAGATGGGAACCTCGATAG |
PCR Primers
| Primer | Sequence |
| SU | CTTGGTTTCAAACTCAGCAC |
| SD | CACTGTGGGCCATCTTAACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16684A-A6 | VGB6 | C57BL/6NTac | |
| 16684A-E3 | VGB6 | C57BL/6NTac | |
| 16684A-H1 | VGB6 | C57BL/6NTac | |
