16688   Clec1b     Status: ES cell colonies screened / QC no positives

GeneClec1b
Chromosome6
Deletion Start129,405,252
Deletion End129,397,618
Deletion Size7,635
Genome Build38
MGI IDMGI:1913287
NCBI Gene ID56760
BAC ID396L6
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableNo

Primer Genomic Coordinates on Chromosome 6

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF129,398,264129,398,283TGCTGAGGGCCATGTAAGAG
TUP129,398,294129,398,322TGGCTTTAGAAGTCTTGGAATGTGGTCCA
TUR129,398,326129,398,345CCCATTGAGGCTGTGCTAAC
TDF129,404,263129,404,284GGACAACAACCATGCAATAGTC
TDP129,404,301129,404,328CCTGATGCTGTTACATCTCAGCTCTGCA
TDR129,404,347129,404,368CATAGAGTCAGCAGCTAGGAAC

PCR Primers

PrimerSequence
SUCCCGCATCTACTCTTTCACC
SDTGCTATCCCATGGTGTCTTTC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR82 bp++-
TDFTDR106 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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