16695 Nrarp Status: Cancelled
| Gene | Nrarp |
| Chromosome | 2 |
| Deletion Start | 25,181,502 |
| Deletion End | 25,181,114 |
| Deletion Size | 389 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 25,181,179 | 25,181,197 | CAAGGGCAACACGCAGGAG |
| TUP | 25,181,199 | 25,181,219 | TGCAGTCGCTGCTGCAGAACA |
| TUR | 25,181,279 | 25,181,297 | TGCCGTCGATGACTGACTG |
| TDF | 25,181,396 | 25,181,417 | CACCAGGACATCGTGCTCTATC |
| TDP | 25,181,424 | 25,181,445 | CCAAGGCCAAGTACGCGGCCAG |
| TDR | 25,181,452 | 25,181,470 | GAGTCCGGGTGGCTATCAC |
PCR Primers
| Primer | Sequence |
| SU | CCTAACCTCGAAGGGACCAC |
| SD | AGGATCCGGTATCTCGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 119 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
