16697 Uty Status: ES cell colonies screened / QC no positives
| Gene | Uty |
| Chromosome | Y |
| Deletion Start | 1,245,549 |
| Deletion End | 1,224,501 |
| Deletion Size | 21,049 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome Y
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 1,244,999 | 1,245,021 | CCGTTTCTCCTTTCTTGGCTTAC |
| TUP | 1,244,974 | 1,244,998 | TTTCCTGATAGTTTGAGGCCCTGCC |
| TUR | 1,244,950 | 1,244,969 | GCAGTGCTCTGGGCTGTATG |
| TDF | 1,225,330 | 1,225,353 | TGGAACTTCAATTACAGGTGGTTG |
| TDP | 1,225,307 | 1,225,329 | TGAGCCACCAGATGTGGGTGCTA |
| TDR | 1,225,274 | 1,225,295 | CATTGCTCATGCCAAGGATCTG |
PCR Primers
| Primer | Sequence |
| SU | AGCTCTTAGGCTGACATCACG |
| SD | CTAAGATACTTTTCATCCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 72 bp | + | + | - |
| TDF | TDR | 80 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
