16726 Foxf1a Status: Cancelled
| Gene | Foxf1a |
| Chromosome | 8 |
| Deletion Start | 121,086,956 |
| Deletion End | 121,084,477 |
| Deletion Size | 2,480 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 121,085,321 | 121,085,340 | TGGAGCAGCCATACCTTCAC |
| TUP | 121,085,343 | 121,085,364 | AAACAGTCACAACGGGCCGGCA |
| TUR | 121,085,365 | 121,085,383 | TGCACTCACCTTGCAGTTC |
| TDF | 121,086,427 | 121,086,451 | GCAGGACTTGGAGTTAGTGATTTAG |
| TDP | 121,086,453 | 121,086,475 | CCAAATCCAGGAAGGCACAGCGA |
| TDR | 121,086,512 | 121,086,533 | GGCAGGAACTGAACTGAGAAAG |
PCR Primers
| Primer | Sequence |
| SU | GGGGATCGGACACTGCAG |
| SD | ATGATTGGTCTCACGTTCCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 63 bp | + | + | - |
| TDF | TDR | 107 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
