| Gene | Eif4ebp3 |
| Chromosome | 18 |
| Deletion Start | 36,665,705 |
| Deletion End | 36,664,203 |
| Deletion Size | 1,503 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 18
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 36,664,767 | 36,664,787 | TGTAGTGGCTGTAGCACTTGG |
| TUP | 36,664,790 | 36,664,813 | CATAAGCTGCTGGGCTGGCAAACA |
| TUR | 36,664,846 | 36,664,864 | GAGCCCTGCCCTGTTCTTG |
| TDF | 36,665,354 | 36,665,372 | GCTGCCTCCCTCAGATTCC |
| TDP | 36,665,378 | 36,665,402 | TCACAACTCTCCCAGCAGTACCACC |
| TDR | 36,665,425 | 36,665,447 | TCTACCTCTGTCTGCTTCTGTTC |
PCR Primers
| Primer | Sequence |
| SU | GATCTTGTCTTCCCGTCCTG |
| SD | GTGGGAGCTGGAAACACTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 98 bp | + | + | - |
| TDF | TDR | 94 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16735A-H10 | VGB6 | C57BL/6NTac | |
| 16735A-E5 | VGB6 | C57BL/6NTac | |
| 16735A-G5 | VGB6 | C57BL/6NTac | |
| 16735A-E12 | VGB6 | C57BL/6NTac | |
| 16735A-F11 | VGB6 | C57BL/6NTac | |
| 16735A-B7 | VGB6 | C57BL/6NTac | |
