| Gene | Slfn1 |
| Chromosome | 11 |
| Deletion Start | 83,122,077 |
| Deletion End | 83,121,063 |
| Deletion Size | 1,015 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 83,121,455 | 83,121,476 | GAACTTGGATGCTGCTCAGAAG |
| TUP | 83,121,485 | 83,121,513 | CAATATCAAGGTTGCAGGAGGAAGGTCGC |
| TUR | 83,121,526 | 83,121,546 | CATCGCCTGGCCTATCTGAAG |
| TDF | 83,121,952 | 83,121,971 | CGCTCAGAGTGGAGAGGTTC |
| TDP | 83,121,975 | 83,121,996 | TGCGCAGTGTTTGCTGCAGAGC |
| TDR | 83,122,001 | 83,122,021 | CCATCTTTCATGTGCCAGGAC |
PCR Primers
| Primer | Sequence |
| SU | TAGGCAAGTGGCTCTTGTGC |
| SD | TTAGGGAGAAAGGAGCATGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 92 bp | + | + | - |
| TDF | TDR | 70 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16752A-H12 | VGB6 | C57BL/6NTac | |
| 16752A-H8 | VGB6 | C57BL/6NTac | |
| 16752A-D7 | VGB6 | C57BL/6NTac | |
| 16752A-C4 | VGB6 | C57BL/6NTac | |
| 16752A-A10 | VGB6 | C57BL/6NTac | |
