16788 Prss35 Status: ES cell colonies screened / QC no positives
| Gene | Prss35 |
| Chromosome | 9 |
| Deletion Start | 86,756,439 |
| Deletion End | 86,755,182 |
| Deletion Size | 1,258 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 86,755,465 | 86,755,485 | CCAGGGTGAAAGTTCAAGGTC |
| TUP | 86,755,486 | 86,755,506 | TGGTCCTGGAACCCACTCGGA |
| TUR | 86,755,548 | 86,755,568 | CCTGCTGTCCGTACCATACAC |
| TDF | 86,756,032 | 86,756,051 | AGCACATGGAGCTGGGAGTC |
| TDP | 86,756,057 | 86,756,079 | CACCATCACCAAGCTGCCGGGAG |
| TDR | 86,756,088 | 86,756,111 | GTCGTTGTCAAATCCAGAGAAGTG |
PCR Primers
| Primer | Sequence |
| SU | TCAGCAGTGGAGCAGTGAAC |
| SD | CCCTGATTCTAACGGTGTTACTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 104 bp | + | + | - |
| TDF | TDR | 80 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
