16807 Ubc Status: Cancelled
| Gene | Ubc |
| Chromosome | 5 |
| Deletion Start | 125,390,118 |
| Deletion End | 125,387,909 |
| Deletion Size | 2,210 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | AGAAAGAGTCCACCCTGCACTTA |
| TUP | | | CCTTCGCCTCAGAGGTGGGATGC |
| TUR | | | GACCTCCAGGGTGATGGTT |
| TDF | | | GGACGTCGAGCCCAGTGTT |
| TDP | | | ACCACCAAGAAGGTCAAACAGGAAGACA |
| TDR | | | CTCCCCCATCACACCCAAGA |
PCR Primers
| Primer | Sequence |
| SU | GGTATACTGAAGTCTCCATC |
| SD | CTACACACTGAGAAACAAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
