16843 Ptpn12 Status: Cancelled
| Gene | Ptpn12 |
| Chromosome | 5 |
| Deletion Start | 21,055,744 |
| Deletion End | 21,022,021 |
| Deletion Size | 33,724 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 21,055,712 | 21,055,733 | GATCCTGAGGAGGTTCATCCAG |
| TUP | 21,055,686 | 21,055,707 | TCCAGGCCATGAAGAGTCCGGA |
| TUR | 21,055,636 | 21,055,657 | AACGGACACTCACCATGAAGTC |
| TDF | 21,022,749 | 21,022,766 | CCAGCAGGCAGCAATGAC |
| TDP | 21,022,723 | 21,022,744 | CCTCCAGAGGACTGGGTCCAGC |
| TDR | 21,022,669 | 21,022,690 | GGGCAATGACCACAGAAGTTAG |
PCR Primers
| Primer | Sequence |
| SU | GCGAACTGAGAGCCGTGT |
| SD | ATTGGAAACTATCATATGGTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 98 bp | + | + | - |
| TDF | TDR | 98 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
