| Gene | Masp1 |
| Chromosome | 16 |
| Deletion Start | 23,520,547 |
| Deletion End | 23,491,926 |
| Deletion Size | 28,622 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 23,519,854 | 23,519,875 | TCAACCTTCCCTGTTTCTTGTG |
| TUP | 23,519,830 | 23,519,853 | TCCCTTCATGTTCCTCACCAGGCA |
| TUR | 23,519,798 | 23,519,819 | GGGTTTCAGACTCTGGTAAAGC |
| TDF | 23,492,332 | 23,492,352 | CCACAGGTGATGGTGGATGAG |
| TDP | 23,492,301 | 23,492,327 | TACCTTCCTTGGAGATTCAGGGTCTCA |
| TDR | 23,492,266 | 23,492,285 | CCAGGAAGCAGGCAGAGAAG |
PCR Primers
| Primer | Sequence |
| SU | ACAGTCTGTCAGGTCCCTCC |
| SD | AGTGCTGAATGTGGCTCAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 87 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16874A-F11 | VGB6 | C57BL/6NTac | |
| 16874A-B4 | VGB6 | C57BL/6NTac | |
| 16874A-C9 | VGB6 | C57BL/6NTac | |
| 16874A-F2 | VGB6 | C57BL/6NTac | |
| 16874A-D5 | VGB6 | C57BL/6NTac | |
| 16874A-D4 | VGB6 | C57BL/6NTac | |
