| Gene | 2700073G19Rik |
| Chromosome | 12 |
| Deletion Start | 87,443,915 |
| Deletion End | 87,443,508 |
| Deletion Size | 408 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 87,443,893 | 87,443,912 | CGAAACCCTGGAGTCGTGAC |
| TUP | 87,443,871 | 87,443,892 | CCCGGAAGAACCTGCCAGAGCC |
| TUR | 87,443,835 | 87,443,854 | CCAACAGTTTGGCCCGGAAG |
| TDF | 87,443,687 | 87,443,704 | GGAGCCGTCATCGACTTC |
| TDP | 87,443,666 | 87,443,686 | TCAGAGGCGCACTTGGCTCGG |
| TDR | 87,443,610 | 87,443,629 | TAGGCTGGCACACACTACTC |
PCR Primers
| Primer | Sequence |
| SU | AGAGTATCAGCATGCGTCCC |
| SD | CCTCAGAGAAGACAGGGTCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 95 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16888A-A9 | VGB6 | C57BL/6NTac | |
| 16888A-D11 | VGB6 | C57BL/6NTac | |
| 16888A-E5 | VGB6 | C57BL/6NTac | |
| 16888A-G9 | VGB6 | C57BL/6NTac | |
| 16888A-F11 | VGB6 | C57BL/6NTac | |
| 16888A-G8 | VGB6 | C57BL/6NTac | |
