16940 Calcrl Status: Cancelled
| Gene | Calcrl |
| Chromosome | 2 |
| Deletion Start | 84,373,353 |
| Deletion End | 84,348,209 |
| Deletion Size | 25,145 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 84,373,288 | 84,373,312 | GACGGCTCAATATGAATGTTACCAG |
| TUP | 84,373,245 | 84,373,271 | CCATTCAACAAGCAGAAGGTAGCTGCA |
| TUR | 84,373,179 | 84,373,201 | AGTAGACGTAAGAACCTGCACAG |
| TDF | 84,348,344 | 84,348,363 | CACAGGTGAGCTGCAAAGTG |
| TDP | 84,348,314 | 84,348,343 | TCTCAGTTTATCCATCTCTACCTGATGGGC |
| TDR | 84,348,282 | 84,348,302 | ACGCCTTCACAGAGCATCCAG |
PCR Primers
| Primer | Sequence |
| SU | GTCAGTCAAGTACCACAGAC |
| SD | ACTAGAGAAACCATGAGCTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 134 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
