17004 N4bp2l1 Status: ES cell colonies screened / QC positives
| Gene | N4bp2l1 |
| Chromosome | 5 |
| Deletion Start | 151,397,008 |
| Deletion End | 151,375,252 |
| Deletion Size | 21,757 |
| Genome Build | 37 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 151,396,149 | 151,396,169 | CCTGCCCTGCACTTGGTATTC |
| TUP | 151,396,115 | 151,396,142 | CGTTGGACTGTTTCTCTCTCCTTGCTGG |
| TUR | 151,396,066 | 151,396,086 | CAAAGGGTGACGGTGGGAAAG |
| TDF | 151,375,747 | 151,375,765 | GCAGGGCAGCATGAGAAAC |
| TDP | 151,375,722 | 151,375,746 | CGCAGCAGAGCTGTCGAAGTACCTT |
| TDR | 151,375,685 | 151,375,705 | CCTCAGGAGACCAGGCAGAAG |
PCR Primers
| Primer | Sequence |
| SU | GCTTCCACAGTGACTGCAG |
| SD | GTGGAGAGGCTGGATCTGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 104 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17004A-B1 | VGB6 | C57BL/6NTac | |
| 17004A-H7 | VGB6 | C57BL/6NTac | |
| 17004A-C8 | VGB6 | C57BL/6NTac | |
| 17004A-C1 | VGB6 | C57BL/6NTac | |
| 17004A-H5 | VGB6 | C57BL/6NTac | |
