17018 Fancl Status: Cancelled
| Gene | Fancl |
| Chromosome | 11 |
| Deletion Start | 26,407,638 |
| Deletion End | 26,387,218 |
| Deletion Size | 20,421 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 26,387,221 | 26,387,241 | GAAGCAGAAGCAAGCCTGTTG |
| TUP | 26,387,242 | 26,387,264 | CGCCATTTCCCGCTGCTACTTCC |
| TUR | 26,387,286 | 26,387,309 | TGAGCCGAAATGAATCCCTCATAC |
| TDF | 26,406,785 | 26,406,808 | ACCCTCTCACTTATCTTGGCTTAG |
| TDP | 26,406,814 | 26,406,836 | TCTATTGTACATTTGGGCTGTGT |
| TDR | 26,406,859 | 26,406,883 | CAATTTCTGAAGAAAGGCAGGCTAC |
PCR Primers
| Primer | Sequence |
| SU | CAGAGCAAACGCAAGAACAG |
| SD | CATTCTTTCAAATCACATGTAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 89 bp | + | + | - |
| TDF | TDR | 99 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
