| Gene | Myh3 |
| Chromosome | 11 |
| Deletion Start | 67,102,178 |
| Deletion End | 67,080,002 |
| Deletion Size | 22,177 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 67,080,155 | 67,080,176 | AAGAGTAGCCAGGATGGGAAAG |
| TUP | 67,080,177 | 67,080,202 | TCACTGTGGAGACAGAAGACAGCAGG |
| TUR | 67,080,215 | 67,080,239 | GGTCAGTGCTGAGAAAGAGAAAGAG |
| TDF | 67,101,608 | 67,101,630 | CGCAGAATCGCAAGTCAATAAAC |
| TDP | 67,101,631 | 67,101,652 | TGCGGGCAAAGACCCGTGACTT |
| TDR | 67,101,693 | 67,101,714 | CCAGCTCTGAAGGGTCTGTATC |
PCR Primers
| Primer | Sequence |
| SU | GGCTGAAAGAATGGAGATGC |
| SD | GTGCAAGCAAGCACACAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 85 bp | + | + | - |
| TDF | TDR | 107 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17019A-H9 | VGB6 | C57BL/6NTac | |
| 17019A-E8 | VGB6 | C57BL/6NTac | |
| 17019A-G9 | VGB6 | C57BL/6NTac | |
| 17019A-E12 | VGB6 | C57BL/6NTac | |
| 17019A-D3 | VGB6 | C57BL/6NTac | |
