17057   Ins2     Status: ES cell clone microinjected

GeneIns2
Chromosome7
Deletion Start142,679,530
Deletion End142,678,625
Deletion Size906
Genome Build38
MGI IDMGI:96573
NCBI Gene ID16334
BAC ID213F7
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 7

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF142,679,510142,679,528CCTGTGGATGCGCTTCCTG
TUP142,679,484142,679,506CTGCTGGCCCTGCTCTTCCTCTG
TUR142,679,437142,679,458AACCACAAAGGTGCTGCTTGAC
TDF142,678,762142,678,785GAAGCGTGGCATTGTAGATCAGTG
TDP142,678,740142,678,760TGCACCAGCATCTGCTCCCTC
TDR142,678,698142,678,719TGGGTAGTGGTGGGTCTAGTTG

PCR Primers

PrimerSequence
SUCAGACCTAGCACCAGGGAAG
SDATCAGCTCCTAGCTGCCATC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR92 bp++-
TDFTDR88 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
17057A-A1VGB6C57BL/6NTac
17057A-B9VGB6C57BL/6NTac
17057A-E1VGB6C57BL/6NTac
17057A-G5VGB6C57BL/6NTac
17057A-G8VGB6C57BL/6NTac
17057A-F2VGB6C57BL/6NTac