17061 Mal2 Status: ES cell colonies screened / QC no positives
| Gene | Mal2 |
| Chromosome | 15 |
| Deletion Start | 54,571,512 |
| Deletion End | 54,600,819 |
| Deletion Size | 29,308 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 54,572,360 | 54,572,377 | GCTCCGCCCAAAGTCAAG |
| TUP | 54,572,381 | 54,572,401 | ATGCCACAAGCCTGCCCTGCC |
| TUR | 54,572,423 | 54,572,446 | CACAGCATTCCTTTCGCTAAGTTC |
| TDF | 54,600,138 | 54,600,157 | GCACAGCGGGTGTTAATGAC |
| TDP | 54,600,159 | 54,600,183 | CCAACCGTGACACCAAGGAAACAGC |
| TDR | 54,600,206 | 54,600,225 | CCTCAGGTGGGAGACAGTTG |
PCR Primers
| Primer | Sequence |
| SU | AGCAAGTGATCTGAAGATCC |
| SD | TGAGAAGCCTGCATGAGATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 87 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
