17064   Ifnar1     Status: ES cell colonies screened / QC no positives

GeneIfnar1
Chromosome16
Deletion Start91,505,501
Deletion End91,485,347
Deletion Size20,155
Genome Build38
MGI IDMGI:107658
NCBI Gene ID15975
BAC ID464C7
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableNo

Primer Genomic Coordinates on Chromosome 16

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF91,486,10491,486,124GTTGGTGGTACTGGAAGTGTG
TUP91,486,12791,486,155TCAGTGGTAGTGTGCACTTAGCATAGACA
TUR91,486,18791,486,207CCAGGAGCTATCCATCAAGTC
TDF91,505,09991,505,119TGAGGAGCACACGGAAAGATG
TDP91,505,12291,505,149TCATCATTGAGAATACAGACACGGTCGC
TDR91,505,18691,505,207GCTGGTCTGTGAGCTGTACTTC

PCR Primers

PrimerSequence
SUAGAGTCATTTGATCCACGGC
SDCAAACACTGACTGGACACGG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR104 bp++-
TDFTDR109 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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