17065 Laptm5 Status: Cancelled
| Gene | Laptm5 |
| Chromosome | 4 |
| Deletion Start | 130,934,547 |
| Deletion End | 130,913,305 |
| Deletion Size | 21,243 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 130,914,105 | 130,914,126 | GAACCTGTGGCTAACAGCTCTC |
| TUP | 130,914,127 | 130,914,149 | CTGGGCCTGGAAGAGCTTCCTCA |
| TUR | 130,914,190 | 130,914,207 | TGGCCGGAAGCAGATGTC |
| TDF | 130,933,975 | 130,933,998 | AACCTCTATCTCTTGACTCTCCTC |
| TDP | 130,933,999 | 130,934,024 | TCCTCAGTCCTGGCTCCAATTTCAGG |
| TDR | 130,934,055 | 130,934,072 | TTGGCCTTTGGGCTCCTG |
PCR Primers
| Primer | Sequence |
| SU | GCTTCACAGGATCTCGAAGG |
| SD | GCCAGATTCTCTTCCTGCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 103 bp | + | + | - |
| TDF | TDR | 98 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
