17070 Ocln Status: ES cell colonies screened / QC positives
| Gene | Ocln |
| Chromosome | 13 |
| Deletion Start | 100,541,114 |
| Deletion End | 100,511,418 |
| Deletion Size | 29,697 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 100,540,501 | 100,540,521 | AGCACGAGGTCCGCATAAGTC |
| TUP | 100,540,478 | 100,540,500 | AGCCTGGCATCTCCAGCAGCCAA |
| TUR | 100,540,420 | 100,540,441 | GCCTAGCATCCTCCTCTTTGAC |
| TDF | 100,511,579 | 100,511,599 | CTGACCTTGAGTGTGGATGAC |
| TDP | 100,511,553 | 100,511,575 | AGGCAGCCTCGGTACAGCAGCAA |
| TDR | 100,511,491 | 100,511,510 | TCTTGCCCTTTCCTGCTTTC |
PCR Primers
| Primer | Sequence |
| SU | AAAGATAACTTGAGTGCCAGGG |
| SD | GAAAGTAACTGCCTCAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 102 bp | + | + | - |
| TDF | TDR | 109 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
