17084   2310067B10Rik     Status: ES cell colonies screened / QC no positives

Gene2310067B10Rik
Chromosome11
Deletion Start115,798,388
Deletion End115,777,357
Deletion Size21,032
Genome Build38
MGI IDMGI:1919197
NCBI Gene ID71947
BAC ID99A10
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableNo

Primer Genomic Coordinates on Chromosome 11

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF115,777,533115,777,553ACGCCTGCTTAGAGGTTAGTG
TUP115,777,554115,777,573TTGCCTGGCTGGGATGCACA
TUR115,777,617115,777,639TGCGATCAAACCCAATCAGAAAG
TDF115,797,547115,797,570TCGCCCATCTTTCTAGTCTTTATC
TDP115,797,571115,797,595TCCATCACTCATGTACACCGCACCA
TDR115,797,623115,797,641ACGGCCCACCAGAGGTTTG

PCR Primers

PrimerSequence
SUAGAATGCCCTACCTGTCTGC
SDACATGGGCAGGAGGATACAG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR107 bp++-
TDFTDR95 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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