Information regarding availability, timelines and delivery of materials can be found at www.komp.org or www.knockoutmouse.org.

17091 Abcb10 Status: ES cell colonies screened / QC positives

Gene	Abcb10
Chromosome	8
Deletion Start	123,982,810
Deletion End	123,954,287
Deletion Size	28,524
Genome Build	38

MGI ID	MGI:1860508
NCBI Gene ID	56199
BAC ID	100H16
BAC Library	bMQ
Cassette	ZEN-Ub1
Clones Available	Yes

Sequence Downloads and Strategy Information

Primer Genomic Coordinates on Chromosome 8

Please be aware that these oligonucleotides have been validated only by Taqman assay.

Primer	Start	End	Sequence
TUF	123,982,017	123,982,035	GAGCTTTGTGCTGGCTGTC
TUP	123,981,987	123,982,013	TCCCTAAGTGGTGACTTGGTCAGGACC
TUR	123,981,938	123,981,958	CTATCCGGCTGAGTTACTGTC
TDF	123,954,510	123,954,529	CGGCTGATGGAAGGGAGAAC
TDP	123,954,486	123,954,507	TGCTGATCATAGCCCACCGCCT
TDR	123,954,444	123,954,464	TGGTCAAGGACAGCAACAAAG

PCR Primers

Primer	Sequence
SU	AGGTTCACAAGTCGTGGAGG
SD	TCAAACTTTCCCTCAGTGGC
LacInR	TTGACTGTAGCGGCTGATGTTG
LacInf	GGTAAACTGGCTCGGATTAGGG
NeoInR	TACTTTCTCGGCAGGAGCAAGGTG
NeoInF	TTCGGCTATGACTGGGCACAACAG
LacInZRev	GTCTGTCCTAGCTTCCTCACTG
NeoFwd	TCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward Primer	Reverse Primer	Length	WT	HET	KO
SU	LacZRev	bp	-	+	+
TUF	TUR	98 bp	+	+	-
TDF	TDR	86 bp	+	+	-
NeoFwd	SD	bp	-	+	+
LacInf	LacInR	210 bp	-	+	+
NeoInf	NeoInR	282 bp	-	+	+

Available Clones

ES Cell ordering information can be found at the KOMP Repository.

Clone Name	Parental ES Cell	Mouse Strain	Cassette Copy Number
17091A-C5	VGB6	C57BL/6NTac
17091B-F7	VGB6	C57BL/6NTac
17091C-F1	VGB6	C57BL/6NTac
17091C-F6	VGB6	C57BL/6NTac