17093 Adam17 Status: ES cell colonies picked
| Gene | Adam17 |
| Chromosome | 12 |
| Deletion Start | 21,358,943 |
| Deletion End | 21,331,140 |
| Deletion Size | 27,804 |
| Genome Build | 37 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 21,378,780 | 21,378,800 | AGTCCCAAAGCTCTCCACCAC |
| TUP | 21,378,751 | 21,378,778 | TGTTGGCTCTCAGAGGCTAACTTGAAGT |
| TUR | 21,378,705 | 21,378,722 | TCGCCACCTTCCAACCAG |
| TDF | 21,351,938 | 21,351,961 | CAGTCATCACCATTTTCCCTGTTC |
| TDP | 21,351,913 | 21,351,936 | AGGGCTACATTTCAGGCACTCGGG |
| TDR | 21,351,893 | 21,351,911 | GCAGTCGGTCGTCACTCTG |
PCR Primers
| Primer | Sequence |
| SU | GGAACAGAGCCCAGAAGATG |
| SD | AGCATTAAGAGGTTTCATTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 96 bp | + | + | - |
| TDF | TDR | 69 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
