| Gene | Cd82 |
| Chromosome | 2 |
| Deletion Start | 93,437,447 |
| Deletion End | 93,419,849 |
| Deletion Size | 17,599 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 93,436,850 | 93,436,869 | GGGTGTGAACAGCCTGAATG |
| TUP | 93,436,819 | 93,436,845 | TCCATTGTCCCAGAGTGCTATTAGGGC |
| TUR | 93,436,792 | 93,436,814 | AGGGTGAATCAGAACAGAGAAGC |
| TDF | 93,420,711 | 93,420,734 | AGCCACTACAACTGGACAGAGAAC |
| TDP | 93,420,683 | 93,420,709 | AGGAGCTCATGGGCTTTACCAAGACCA |
| TDR | 93,420,664 | 93,420,682 | TCGCAGGAGCATGGGTAAG |
PCR Primers
| Primer | Sequence |
| SU | AACATGGAAGGCAGGCACAG |
| SD | TGGAAGAATTGCCTACCCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 71 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17102A-C1 | VGB6 | C57BL/6NTac | |
| 17102A-F9 | VGB6 | C57BL/6NTac | |
| 17102A-G8 | VGB6 | C57BL/6NTac | |
