17104 Cdh1 Status: ES cell colonies screened / QC no positives
| Gene | Cdh1 |
| Chromosome | 8 |
| Deletion Start | 106,604,403 |
| Deletion End | 106,603,498 |
| Deletion Size | 906 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 106,603,835 | 106,603,853 | GGCCTGCCTGTAGAAGGTG |
| TUP | 106,603,859 | 106,603,881 | CTGAAGTAGCGCGGACGATCCGA |
| TUR | 106,603,913 | 106,603,933 | CCCTCCCTAACAGGACAGGTG |
| TDF | 106,604,270 | 106,604,291 | CTTTACCTCCATCCCAGGTCTC |
| TDP | 106,604,299 | 106,604,321 | CTTTGCCAGGAGCTGGAGCCTGA |
| TDR | 106,604,328 | 106,604,346 | GGAACTGAAGCCGGGACTG |
PCR Primers
| Primer | Sequence |
| SU | TAGGAAGCTGGGAAGTCTTC |
| SD | GCTCCAGTAATCAGTTGGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 99 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
