17105 Cdh3 Status: ES cell colonies screened / QC positives
| Gene | Cdh3 |
| Chromosome | 8 |
| Deletion Start | 106,539,164 |
| Deletion End | 106,510,962 |
| Deletion Size | 28,203 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 106,511,198 | 106,511,219 | TTTAACCCTTCTACCCTCACAC |
| TUP | 106,511,220 | 106,511,241 | TCAGGTTTGCTGGCTACGCAGC |
| TUR | 106,511,285 | 106,511,304 | TTCCACCTCCAAGGTCACTC |
| TDF | 106,538,988 | 106,539,007 | CGGCAGGCACTTCTGTGATG |
| TDP | 106,539,008 | 106,539,034 | CAGGTGACAGCCACAGATGAGGACGAT |
| TDR | 106,539,070 | 106,539,089 | TCGGCTCTTGGCTATGGATG |
PCR Primers
| Primer | Sequence |
| SU | TTTCCATCGAAGCCAGAGAC |
| SD | CGTTCGGACAGGAAATGAGA |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 107 bp | + | + | - |
| TDF | TDR | 102 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
