17108 Chrna4 Status: ES cell colonies screened / QC positives
| Gene | Chrna4 |
| Chromosome | 2 |
| Deletion Start | 180,773,867 |
| Deletion End | 180,759,462 |
| Deletion Size | 14,406 |
| Genome Build | 37 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 180,773,425 | 180,773,445 | GCATTTCGGAGCTGGAATCAG |
| TUP | 180,773,393 | 180,773,421 | CTCCAAGTAGGATAGAGGTGGAGTCCACC |
| TUR | 180,773,358 | 180,773,378 | AAGGCGAGATTTGGCTGGATG |
| TDF | 180,759,573 | 180,759,591 | TTTGCAGGTGAAGGAGGAC |
| TDP | 180,759,543 | 180,759,569 | AAATACGTGGCCATGGTCATTGACCGA |
| TDR | 180,759,505 | 180,759,528 | CCCAGAAGGCAGACAATGATGAAC |
PCR Primers
| Primer | Sequence |
| SU | CCGAGATGACTGGAATGACC |
| SD | TTTGAGTCAGAGAGCAGCCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 87 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17108A-A3 | VGB6 | C57BL/6NTac | |
| 17108A-E6 | VGB6 | C57BL/6NTac | |
| 17108A-E11 | VGB6 | C57BL/6NTac | |
| 17108A-E10 | VGB6 | C57BL/6NTac | |
| 17108A-G2 | VGB6 | C57BL/6NTac | |
