17110 Chrng Status: Cancelled
| Gene | Chrng |
| Chromosome | 1 |
| Deletion Start | 87,211,510 |
| Deletion End | 87,205,828 |
| Deletion Size | 5,683 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 87,206,477 | 87,206,497 | GAGGAGGCCCTCACAACTAAC |
| TUP | 87,206,499 | 87,206,525 | TCTGGATAGAGATGGTAAGACGCCACC |
| TUR | 87,206,535 | 87,206,554 | GCAGCCGCAGTAAGCAGAAC |
| TDF | 87,211,368 | 87,211,386 | AGCCATGCTCTCCCTCTTC |
| TDP | 87,211,388 | 87,211,412 | TCTGTGGCACTGCTGGCATCTTCCT |
| TDR | 87,211,422 | 87,211,441 | GGTCAGGCACTTGGTTGTAG |
PCR Primers
| Primer | Sequence |
| SU | AGGGTGTCCTGTCTCTGGTG |
| SD | AGTCAATGACATCAGGAAAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
