17112 Clca5 Status: Cancelled
| Gene | Clca5 |
| Chromosome | 3 |
| Deletion Start | 145,098,961 |
| Deletion End | 145,071,245 |
| Deletion Size | 27,717 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 145,098,915 | 145,098,935 | CATCGGGCTGAAACTTGTGAC |
| TUP | 145,098,880 | 145,098,909 | TGTTCACCCTAAGTCCAGAACTTCTGTTCC |
| TUR | 145,098,832 | 145,098,856 | CAAGCAATCCATCATAGCCATTCTC |
| TDF | 145,071,510 | 145,071,532 | CATAGTGTATGTGGCCCTGAGAG |
| TDP | 145,071,482 | 145,071,508 | CATGGATAGAAGCTCCCTCAGGTCAGC |
| TDR | 145,071,429 | 145,071,452 | CAGGAGAAGAGTTTGGAGGAAGAG |
PCR Primers
| Primer | Sequence |
| SU | AGCTGTGATGCAGGAGGAAG |
| SD | AAACATGTGTCCTTCTCTGCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 104 bp | + | + | - |
| TDF | TDR | 104 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
