17127   Ifitm6     Status: ES cell colonies screened / QC positives

GeneIfitm6
Chromosome7
Deletion Start141,016,855
Deletion End141,015,902
Deletion Size954
Genome Build38
MGI IDMGI:2686976
NCBI Gene ID213002
BAC ID160A5
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 7

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF141,016,724141,016,743ATGGCTGCTGCCTGGGTTTC
TUP141,016,696141,016,721TGCCTACATCTACTCGGTGAAGGTGA
TUR141,016,659141,016,679GGAGGAGCCACAACCACATAC
TDF141,016,179141,016,196TGCTCCAAGGCCACACTG
TDP141,016,148141,016,174TTTCATCTCTTTCAGTCCAGGGACCGG
TDR141,016,101141,016,120GCGGTTGAAGCATGGGATTG

PCR Primers

PrimerSequence
SUGCCCTTCTTGAACCCTATCC
SDATGCCCTGCACTATACACCC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR85 bp++-
TDFTDR96 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
17127A-D5VGB6C57BL/6NTac
17127A-G5VGB6C57BL/6NTac
17127A-C8VGB6C57BL/6NTac
17127A-E11VGB6C57BL/6NTac
17127A-D7VGB6C57BL/6NTac
17127A-D8VGB6C57BL/6NTac