17144 Kcne4 Status: ES cell colonies screened / QC no positives
| Gene | Kcne4 |
| Chromosome | 1 |
| Deletion Start | 78,818,251 |
| Deletion End | 78,817,640 |
| Deletion Size | 612 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 78,817,786 | 78,817,807 | GATCGGAATCATGCTGGGCTAC |
| TUP | 78,817,811 | 78,817,838 | AAATCCAAGAGGCGGGAGAAGAAGTCCA |
| TUR | 78,817,855 | 78,817,874 | ACAGCCTCTCCTCGTCTTTG |
| TDF | 78,818,113 | 78,818,134 | TGAAGGCTCTTCCGAGAACATC |
| TDP | 78,818,135 | 78,818,160 | CACCAGAATTCCTAGCACCCACCAGG |
| TDR | 78,818,178 | 78,818,200 | TCCCTCTGTCAAGTGTAGCTTAC |
PCR Primers
| Primer | Sequence |
| SU | CTGCCGTGCCAGCTAAAG |
| SD | ATCGAAACGAGTCCTTCAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 89 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
