| Gene | Kcnh6 |
| Chromosome | 11 |
| Deletion Start | 106,034,112 |
| Deletion End | 106,008,206 |
| Deletion Size | 25,907 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 106,009,060 | 106,009,077 | TTCGGCTACTCCCGTGTG |
| TUP | 106,009,079 | 106,009,101 | AGGTGATGCAGCGACCCTGTACC |
| TUR | 106,009,111 | 106,009,129 | GAGTGTTCGGGCCAGTGAG |
| TDF | 106,033,858 | 106,033,876 | GCGGCAATGGACAAATCTC |
| TDP | 106,033,877 | 106,033,900 | TGGTGCCGTCCTCAGAACAAGAGC |
| TDR | 106,033,915 | 106,033,932 | TGAGGCCAGAGGTGACAG |
PCR Primers
| Primer | Sequence |
| SU | CCTTGGCTTATTTCAGCACC |
| SD | CTTGGGTCTGAGCTTTGGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 70 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17147A-A9 | VGB6 | C57BL/6NTac | |
| 17147A-B6 | VGB6 | C57BL/6NTac | |
| 17147A-D10 | VGB6 | C57BL/6NTac | |
| 17147A-H2 | VGB6 | C57BL/6NTac | |
| 17147A-G12 | VGB6 | C57BL/6NTac | |
| 17147A-E3 | VGB6 | C57BL/6NTac | |
