| Gene | Kcnj12 |
| Chromosome | 11 |
| Deletion Start | 61,070,500 |
| Deletion End | 61,069,187 |
| Deletion Size | 1,314 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 61,069,320 | 61,069,341 | TCGTCAAGAAGAACGGTCAGTG |
| TUP | 61,069,342 | 61,069,370 | CAACATTGAATTCGCCAACATGGACGAGA |
| TUR | 61,069,374 | 61,069,392 | TCAGCCAGGTAGCGTTGTG |
| TDF | 61,070,387 | 61,070,405 | CAGCCCGAGCATGACTTTG |
| TDP | 61,070,408 | 61,070,428 | AGACTGCAGGCCAGCAGCGCT |
| TDR | 61,070,466 | 61,070,488 | CTGCATCTAAGCCAAGGGCATTC |
PCR Primers
| Primer | Sequence |
| SU | TGAATTGAGCTGGTGAGACG |
| SD | CATACACAGCCTCCTCCCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 102 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17148A-H7 | VGB6 | C57BL/6NTac | |
| 17148A-B7 | VGB6 | C57BL/6NTac | |
| 17148A-D4 | VGB6 | C57BL/6NTac | |
| 17148A-D6 | VGB6 | C57BL/6NTac | |
| 17148A-B3 | VGB6 | C57BL/6NTac | |
