17150 Kcnq4 Status: Cancelled
| Gene | Kcnq4 |
| Chromosome | 4 |
| Deletion Start | 120,747,173 |
| Deletion End | 120,716,930 |
| Deletion Size | 30,244 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | GTTTCCAACTTTATCCGTGTCTTC |
| TUP | | | TTAGTCTTGCTCCGCACCCTCAAC |
| TUR | | | CCTGCTGCCTTGGGTACTTC |
| TDF | | | CTGCACCCGAACGGAAAC |
| TDP | | | ACCTCACCAACAGAAGGCTTTCCACCA |
| TDR | | | TGGCATTGAACATGGCTCTC |
PCR Primers
| Primer | Sequence |
| SU | GGACACACCGACTGGGCGGAC |
| SD | GATCCATACGCACCATGCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
