| Gene | Lrig1 |
| Chromosome | 6 |
| Deletion Start | 94,699,682 |
| Deletion End | 94,663,932 |
| Deletion Size | 35,751 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 94,699,034 | 94,699,051 | GGACGCAATGGCAGAGTG |
| TUP | 94,698,995 | 94,699,023 | TGTTGGGATTGGGATCCATGGCTGTATCT |
| TUR | 94,698,959 | 94,698,981 | CACCTAAGGGCTGGGTTACATAC |
| TDF | 94,664,326 | 94,664,346 | GAGTGGCCCTGGACTTGATAC |
| TDP | 94,664,293 | 94,664,322 | ACAAAGCATTTGGATTAGTACTGGCCCTTC |
| TDR | 94,664,261 | 94,664,280 | ACCAACTGGCTAACCAACAC |
PCR Primers
| Primer | Sequence |
| SU | CACGGCTATCATCAGCTCAG |
| SD | GCGAATTTGATCTCTTACAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 93 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17154A-A3 | VGB6 | C57BL/6NTac | |
| 17154B-D2 | VGB6 | C57BL/6NTac | |
| 17154B-G11 | VGB6 | C57BL/6NTac | |
| 17154B-E6 | VGB6 | C57BL/6NTac | |
