17165 Mrps9 Status: ES cell colonies screened / QC no positives
| Gene | Mrps9 |
| Chromosome | 1 |
| Deletion Start | 42,862,645 |
| Deletion End | 42,851,295 |
| Deletion Size | 11,351 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 42,851,320 | 42,851,341 | GAGTGCTTTCCCTTTGGTTCAC |
| TUP | 42,851,343 | 42,851,366 | CCCGCTGTTAGAGCTAGCCTCTGC |
| TUR | 42,851,427 | 42,851,447 | CTCCAGCTCCCAGGACCTTAC |
| TDF | 42,861,900 | 42,861,921 | TCAAACAGGGAGTACCCACAAG |
| TDP | 42,861,923 | 42,861,950 | CCACAGCACTATCCTCAACCCTTGTGTC |
| TDR | 42,861,964 | 42,861,987 | TCTCACGATACAAACAGGCAAATG |
PCR Primers
| Primer | Sequence |
| SU | AAAGCCAGCTTGGATTGTTG |
| SD | GAAGAACATGGCAAAGACAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 128 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
