| Gene | Serping1 |
| Chromosome | 2 |
| Deletion Start | 84,774,787 |
| Deletion End | 84,765,476 |
| Deletion Size | 9,312 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 84,774,275 | 84,774,296 | AGGACAGGGATAACAAGTCAAC |
| TUP | 84,774,242 | 84,774,268 | AAACTGTGAGCGCTTGCTTACGGTGGA |
| TUR | 84,774,213 | 84,774,232 | TGCCAGCTTCCAGCATAGTC |
| TDF | 84,765,643 | 84,765,660 | GTGCAGCGACCTTTCCTC |
| TDP | 84,765,619 | 84,765,641 | TCCTGCTCTGGGACCAGCAACAC |
| TDR | 84,765,561 | 84,765,579 | ACCCAAGCCTGTCTCAACC |
PCR Primers
| Primer | Sequence |
| SU | GTTCTGTTAAGCGGAGCAGG |
| SD | AACCAACCCACTCACGATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17175A-A8 | VGB6 | C57BL/6NTac | |
| 17175A-G1 | VGB6 | C57BL/6NTac | |
| 17175A-B2 | VGB6 | C57BL/6NTac | |
| 17175A-E2 | VGB6 | C57BL/6NTac | |
| 17175A-F10 | VGB6 | C57BL/6NTac | |
